I love the way my "old" pre-Lion version of 1Password allows me to unlock it using the Master Password, and it stays unlocked across reboots/restarts, switching users, etc.
Not so the Lion version from the Mac App Store! It seems it is always asking me to enter the Master Password whenever I restart/reboot, switch users, etc. And after unlocking it, it seems that I'll try to use it just to find it has relocked itself.
I find it a royal PITA compared to the previous version. (Yes, I've got "universal unlock" working, but that doesn't address the issue of it universally relocking itself!)
I own both Snow Leopard & Lion versions on separate computers, so every time I use the current Lion version, I'm reminded of how irritating it is.